Mutation detection in familial and sporadic breast cancers by denaturing high-performance liquid chromatography (DHPLC)
نویسندگان
چکیده
منابع مشابه
Denaturing high-performance liquid chromatography for mutation detection and genotyping.
Denaturing high-performance liquid chromatography (DHPLC) is an accurate and efficient screening technique used for detecting DNA sequence changes by heteroduplex analysis. It can also be used for genotyping of single nucleotide polymorphisms (SNPs). The high sensitivity of DHPLC has made this technique one of the most reliable approaches to mutation analysis and, therefore, used in various are...
متن کاملDenaturing high performance liquid chromatography (DHPLC) used in the detection of germline and somatic mutations.
Denaturing high performance liquid chromatography (DHPLC) has been described recently as a method for screening DNA samples for single nucleotide polymorphisms and inherited mutations. Thirty-eight DNAs, 22 of which were heterozygous for previously characterized rearranged transforming gene (RET) or cystic fibrosis transmembrane conductance regulator gene (CFTR) mutations or polymorphisms, were...
متن کاملDetection of CYP2C18 m1, and m2 Alleles within an Iranian Population (Mazandaran) Using Denaturing High-Performance Liquid Chromatography (DHPLC)
Background: Genetic polymorphisms of cytochrome p450 in humans are the main cause of differences in the metabolism. The allele and genotype frequencies of CYP2C19 and CYP2C9 have been studied in some Iranian populations. The aim of present study was to examine the frequencies of CYP2C18m1, and CYP2C18m2, alleles in the Mazandarani ethnic group among Iranian Population. Materials and Methods: I...
متن کاملmutation detection in human estrogen receptor ? gene in infertile male patients by denaturing high-performance liquid chromatography
background: for screening sequence variations in genes, rapid turnover time is of fundamental importance. while, many of the current methods are unfortunately time consuming and technically difficult to implement. denaturing high-performance liquid chromatography (dhplc) method had been shown to be a high-throughput, time saving, and economical tool for mutation screening. objective: in the pre...
متن کاملdetection of cyp2c18 m1, and m2 alleles within an iranian population (mazandaran) using denaturing high-performance liquid chromatography (dhplc)
background: genetic polymorphisms of cytochrome p450 in humans are the main cause of differences in the metabolism. the allele and genotype frequencies of cyp2c19 and cyp2c9 have been studied in some iranian populations. the aim of present study was to examine the frequencies of cyp2c18m1, and cyp2c18m2, alleles in the mazandarani ethnic group among iranian population. materials and methods: in...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Breast Cancer Research
سال: 2001
ISSN: 1465-542X
DOI: 10.1186/bcr409